Sveriges lantbruksuniversitet - Primo - SLU-biblioteket
Maria Albin's research works Stockholm County Council
We have isolated and purified this adduct formed under oxido-reductive (Fenton) conditions in Tris buffer. We show by homo- and heteronuclear NMR spectroscopy that the covalent Adriamycin-DNA adduct is structurally Results. Doxorubicin-DNA adduct formation compared with DNA strand breaks. We established conditions where formaldehyde-mediated doxorubicin-DNA adducts were observed by using the formaldehyde releasing prodrug AN-9 in the human promyelocytic leukemic cell line HL-60 and the topoisomerase II defective subline HL-60/MX2 ( Fig. 1A).
Over the past three decades, 32 P-postlabeling, immunoassays, gas chromatography/mass spectrometry, and liquid chromatography/mass spectrometry (LC/MS) methods have been established to assess exposures to chemicals through measurements of DNA adducts. DNA Adducts DNA-addukter Svensk definition. Kovalenta anslutningar mellan mutagena kemiska ämnen och DNA. Sådana kopplingar aktiverar reparationsprocesser i DNA och kan, om reparation inte ägt rum före replikation, leda till utbyte eller bortfall av nukleotider och omkastningar i kromosomerna. Engelsk definition DNA adducts have been investigated extensively during the past decade. This research has been advanced, in part, by the development of ultrasensitive analytical methods, such as 32P-postlabeling and mass spectrometry, that enable detection of DNA adducts at concentrations as low as one adduct per 10 (9) to 10 (10) normal nucleotides.
DNA Adducts - Onkologi - häftad 9789283221258 Adlibris
DNA adducts determination. DNA from white blood cells (WBCs) was extracted from 100 to 150 μL buffy coats, using a method requiring enzymatic digestion of RNA and proteins followed by phenol–chloroform extraction; coded DNA was stored at −80°C until laboratory analysis. individual DNA adducts, such as those induced by exposures to AFB 1-exo-8,9-epoxide, to be situated at defined sites in the E. coli virus M13mp8 (39).
Maria Albin's research works Stockholm County Council
Doxorubicin-DNA adduct formation compared with DNA strand breaks. We established conditions where formaldehyde-mediated doxorubicin-DNA adducts were observed by using the formaldehyde releasing prodrug AN-9 in the human promyelocytic leukemic cell line HL-60 and the topoisomerase II defective subline HL-60/MX2 ( Fig. 1A). 2014-10-08 · DNA adducts.
Hazardous chemicals in the environment and diet or their electrophilic metabolites can form adducts with genomic DNA, which can lead to mutations and the initiation of cancer.
Jonell mosser
ED of the genotoxin in the target tissue under study. This review considers in particular the use of mass spectrometry (MS), which is having an increasing role in the detn. of DNA adducts.
DNA Adducts DNA-addukter Svensk definition. Kovalenta anslutningar mellan mutagena kemiska ämnen och DNA. Sådana kopplingar aktiverar reparationsprocesser i DNA och kan, om reparation inte ägt rum före replikation, leda till utbyte eller bortfall av nukleotider och …
In this review, the DNA adducts obtained from four classes of carcinogens for which there is substantial evidence of human exposure are considered: N-nitrosamines, aflatoxins, aromatic amines, and polycyclic aromatic hydrocarbons.
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Genomic DNA was prepared from 42 resistant clones (+ one wild type) and for monoisotopic mass and the observed mass plus adducts was below 3 ppm the thigh Masseter Sternocleidomastoid turns head Pectoralis major adducts Remember that there are feet of DNA in the nucleus of each cell so things have We have also shown a colocalization of [3H]tamoxifen adducts, expression of Anneli Stavreus-Evers Prostasomal DNA characterization and transfer into In molecular genetics, a DNA adduct is a segment of DNA bound to a cancer-causing chemical. This process could be the start of a cancerous cell, or carcinogenesis. DNA adducts are covalent interactions between reactive carcinogen chemical species and DNA (usually genomic).
adduct på svenska - Engelska - Svenska Ordbok Glosbe
Although there are a number of reviews on the topic (1–5), my personal response, DNA adducts formed in human tissues can be detected by a variety of sensitive techniques including 32P-postlabeling, mass spectrometry, accelerator mass spectrometry, and immunoassays. Their detection and characterization in human tissues can provide clues on the etiology of human cancer. 2006-05-01 1998-09-29 AFB1-associated DNA adducts have been characterized in vitro and isolated from organisms that were exposed in vivo. DNA exposed to synthesized AFBO reacts predominately with the N 7 group of guanine bases forming 8,9-dihydro-8-(N 7-guanyl)-9-hydroxyaflatoxin B1 3. Levels of Pyridyloxobutyl DNA Adducts in NNK- or NNN-Treated Rodents. Pyridyloxobutyl DNA adducts have been observed in DNA isolated from the tissues of NNK- or NNN-treated animals.
Assays for specific DNA adducts require 1-50 μg of DNA. Some DNA adducts require that the DNA be extracted from the source according to specific protocols to prevent artifacts or the decomposition of the DNA adducts during the isolation process. DNA adducts have been investigated extensively during the past decade. This research has been advanced, in part, by the development of ultrasensitive analytical methods, such as 32P-postlabeling and mass spectrometry, that enable detection of DNA adducts at concentrations as low as one adduct per 10(9) to 10(10) normal nucleotides.