When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.
Invitrogen Anti-Ku70/Ku80 Monoclonal (162), Catalog # MA1-21818. Tested in Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)) and Dec 5, 2006 Accumulation of XRCC4/ligase IV on DSBs depended on the presence of Ku70/ 80, but not DNA-PKCS. We detected a direct interaction between Feb 27, 2014 The KU70-deficient mutant generated herein was effective in improving gene deletion frequency and allowed shorter homology sequences to Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area. Jul 24, 2015 ARABIDOPSIS THALIANA KU70 HOMOLOG, ATKU70, KU70, KU70 Ku80 and ku70 form the heterodimer complex Ku, required for proper May 1, 2013 The core NHEJ machinery includes the end-binding heterodimeric proteins Ku70 /Ku80, the DNA-PKcs protein kinase, and the complex Jun 20, 2012 The Ku heterodimer complex consists of Ku70 and Ku80 and is a very versatile protein that regulates a wide range of important and diverse Ku70 Antibody (E-5) is a monoclonal anti-KU70 antibody that detects m, r, and h Ku70 by WB, IP, IF, IHC(P) and ELISA. Cited in 60 publications. Jun 22, 2010 Ku70 and Ku80 are part of a high‐affinity heterodimeric protein complex, known as Ku (19, 27).
Ku70 is required for late B cell development and immunoglobulin heavy chain class switching Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement. Eukaryotic Ku is a heterodimer of two polypeptides, Ku70 (XRCC6) and Ku80 (XRCC5), so named because the molecular weight of the human Ku proteins is around 70 kDa and 80 kDa.
Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (1 mg/mL). Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
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On the basis of their predicted sequence similarity to human Ku70 and Ku80, cDNAs encoding the first plant homologues of these proteins (AtKu70 and AtKu80, respectively) have now been isolated from Arabidopsis thaliana. AtKu70 and AtKu80 share 28.6 and 22.5% amino acid sequence identity with human Ku70 and Ku80, respectively. Fig. 1. Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice. (A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm.
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The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). KU70 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human, rat samples. Cat.No.
Cited in 11 reference(s). Independently reviewed in 1
Unconjugated Whole IgG Rabbit anti-Ku70 Antibody, Affinity Purified suitable for WB, IP applications. Visit Bethyl.com for all your antibody needs. Ku70 and Ku80 participate in LPS-induced pro-inflammatory cytokines production in human macrophages and monocytes.
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They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes. The stability of these proteins depends on each other. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] .
Kom ihåg att KU70 inte behöver lämnas då den som mottar näringsbidraget är en juridisk person.
Ku70 + Ku80 (2) Ku70+Ku80+DNA Ligase IV/LIG4+Histone H2A.X+gamma H2A.X+Double strand break repair – Non Homologous E (1) Applications Clear 2007-05-25 Ku70 acetylation and enhances DNA repair activity, suggest-ing that Ku70 acetylation may have an inhibitory role in DNA repair.12 Several studies, however, have demonstrated that the level of Ku70 correlates with radiosensitivity.13 Ku70 knock down increases … KU70 – Kontrolluppgift – Näringsbidrag och royalty (SKV 2316) På skatteverket.se använder vi kakor (cookies) för att webbplatsen ska fungera på ett bra sätt för dig. Genom att surfa vidare godkänner du att vi använder kakor. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V(D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system. Royaltyn eller avgiften ska då i stället redovisas på KU10.